LOCUS       sequence-18496-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  TITLE     NFAT plasmids
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pENTR11 WT NFAT1
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    62..86
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             131..220
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 tttttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct tttttataat
       61 gccaactttg tacaaaaaag caggcttcga aggagataga accaattctt aagcttccac
      121 catgatcttt tacccatacg atgttcctga ctatgcgggc tatccctatg acgtcccgga
      181 ctatgcagga tcctatccat atgacgttcc agattacgct gctcagtgcg gccgcgacgt
      241 cccggagccg cagcccgacc ccgatggcgg ggacggcccc ggccacgagc ccgggggcag
      301 tccccaagac gagctggact tttccatcct cttcgattat gactatctga accctatcga
      361 agaagaaccg atcgcacata aggccatcag ctcaccctcc ggactcgcat acccggatga
      421 tgtcctggac tatggcctca agccatgcaa cccccttgcc agtctctctg gcgagccccc
      481 tggccggttc ggagagccgg atagtatagg gttccagaac tttctgagcc cggtcaagcc
      541 agcaggggct tcgggcccga gccctcggat cgagatcact ccatcccacg aactgatgca
      601 ggcagggggg gccctccgtg ggagagacgc cggcctgtcc cccgagcagc cggccctggc
      661 cctggccggc gtggccgcca gcccgaggtt cacactgccc gtgcccggct acganggcta
      721 ccgcgagccg ctttgcttga gccccgctag cagcggctcc tctgccagct tcatttctga
      781 caccttctcc ccctacacct cgccctgcgt ctcacccaa
//
