LOCUS       sequence-18499-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Muzio M, Salvesen GS, Dixit VM
  TITLE     FLICE induced apoptosis in a cell-free system. Cleavage of caspase 
            zymogens.
  JOURNAL   J Biol Chem. 1997 Jan 31. 272(5):2952-6.
  PUBMED    9006941
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1997 Jan 31. 272(5):2952-6."
COMMENT     Sequence Label: pFLAG-CrmA
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(387..404)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(417..439)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    428..444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(452..458,459..474,475..480))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     primer_bind     complement(727..746)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gctatgcngg annnggtatc tggagacaat ggttcatcca atagtggatt aattttcccc
       61 tcagtgaaga tatcaacaca cttgttgatc gcatctacag tgcgacaatc agtgaagtca
      121 acagtttgga aattatctcc aatttttctc aaaaaggaat ctttaaacac tgcagaatat
      181 cgcccatata ctttattcat ggacttgaat gagatatcat ccttattctt gtccgcctcc
      241 ttttctacat attttgatag ctgttcagca gtggatccat tagctccata atacagtatt
      301 gtcaatactg acgagattga cggtggagaa atgaatacat tctctccttt catagaagat
      361 gcgatttccc tgaagatatc catggagtgg tggtgatgat gatgcatatg atatctcctg
      421 tgtgaaattg ttatccgctc acaattccac acattatacg agccgatgat taattgtcaa
      481 cagctcattt cagaatattt gccagaaccg ttatgatgtc ggcgcaaaaa acattatcca
      541 gaacgggagt gcgccttgag cgacacgaat tatgcagtga tttacgacct gcacagccat
      601 accacagctt ccgatggctg cctgacgcca gaagcattgg tgcaccgtgc agtcgataag
      661 cccggatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggngcggntg
      721 cgggcnccta tatcgccgac atcaccgatg gggaagatcg gctcgccnnt gggccncang
      781 acnctgtncc gcggggtatg gggcagcccc gtgccgggan tgtgggcgcc tctgcncg
//
