LOCUS       sequence-18510-e         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Stennicke HR, Deveraux QL, Humke EW, Reed JC, Dixit VM, Salvesen GS
  TITLE     Caspase-9 can be activated without proteolytic processing.
  JOURNAL   J Biol Chem. 1999 Mar 26. 274(13):8359-62.
  PUBMED    10085063
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Mar 26. 274(13):8359-62."
COMMENT     Sequence Label: pET23b-Casp9-His
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..44)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(57..74)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             582..593
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgct agattagtgg
       61 tggtggtggt ggtgtgatgt tttaaagaaa agttttttcc ggaggaaatt aaagcaacca
      121 ggcatctgtt tataaatccc tttcaccgaa acagcattag cgaccctaag caggagggac
      181 tgcaggtctt cagagtgagc ccactgctca aagatgtcgt ccagggtctc aacgtaccag
      241 gagccactct tggggtccct ccaggaaaca aaacctggga aagtagagta ggacacaaag
      301 atgtcactgg gtgtgggcaa actagatatg gcgtccagct ggtcgaaggt cctcaaacct
      361 tcctggaacg gggtggcatc tggctcgggg ttactgccag gggactcgtc ttcaggggaa
      421 gtggaggcca cctcaaaccc atggtctttc tgctccccac cacaggcctg gatgaaaaag
      481 agcttgggct tccctcccag gctggggcag ctggtcccat tgaagatgtt cacaatcttc
      541 tcgaccgaca cagggcatcc atctgtgccg tagacagccc ctgggaactg caggtggctg
      601 gcctgacagc cgtgagagag aatgaccacc acgcagcagt ccagagcacc gtggtcctgc
      661 cgcgccagc
//
