LOCUS       sequence-18511-e         617 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Stennicke HR, Deveraux QL, Humke EW, Reed JC, Dixit VM, Salvesen GS
  TITLE     Caspase-9 can be activated without proteolytic processing.
  JOURNAL   J Biol Chem. 1999 Mar 26. 274(13):8359-62.
  PUBMED    10085063
REFERENCE   2  (bases 1 to 617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Mar 26. 274(13):8359-62."
COMMENT     Sequence Label: pET23b-Casp9 C287A-His
FEATURES             Location/Qualifiers
     source          1..617
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..45)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(58..75)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             583..594
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 tcgggctttg ttagcagccg gatctcagtg gtggtggtgg tggtgctcgc tagattagtg
       61 gtggtggtgg tggtgtgatg ttttaaagaa aagttttttc cggaggaaat taaagcaacc
      121 aggcatctgt ttataaatcc ctttcaccga aacagcatta gcgaccctaa gcaggaggga
      181 ctgcaggtct tcagagtgag cccactgctc aaagatgtcg tccagggtct caacgtacca
      241 ggagccactc ttggggtccc tccaggaaac aaaacctggg aaagtagagt aggacacaaa
      301 gatgtcactg ggtgtgggca aactagatat ggcgtccagc tggtcgaagg tcctcaaacc
      361 ttcctggaac ggggtggcat ctggctcggg gttactgcca ggggactcgt cttcagggga
      421 agtggaggcc acctcaaacc catggtcttt ctgctcccca cccgcggcct ggatgaaaaa
      481 gagcttgggc ttccctccca ggctggggca gctggtccca ttgaagatgt tcacaatctt
      541 ctcgaccgac acagggcatc catctgtgcc gtagacagcc cctgggaact gcaggtggct
      601 ggcctgacag ccgtgag
//
