LOCUS       sequence-18525-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Wilker EW, Grant RA, Artim SC, Yaffe MB
  TITLE     A structural basis for 14-3-3sigma functional specificity.
  JOURNAL   J Biol Chem. 2005 May 13. 280(19):18891-8.
  PUBMED    15731107
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 May 13. 280(19):18891-8."
COMMENT     Sequence Label: pcDNA3.1/HisC 14-3-3 sigma
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..49
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             53..85
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(89..97,98..112)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 gtttaactta agcttaccat gggggggttc tcatcatcat catcatcatg gtatggctag
       61 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggtaccagg
      121 atccgagaga gccagtctga tccagaaggc caagctggca gagcaggccg aacgctatga
      181 ggacatggca gccttcatga aaggcgccgt ggagaagggc gaggagctct cctgcgaaga
      241 gcgaaacctg ctctcagtag cctataagaa cgtggtgggc ggccagaggg ctgcctggag
      301 ggtgctgtcc agtattgagc agaaaagcaa cgaggagggc tcggaggaga aggggcccga
      361 ggtgcgtgag taccgggaga aggtggagac tgagctccag ggcgtgtgcg acaccgtgct
      421 gggcctgctg gacagccacc tcatcaagga ggccggggac gccgagagcc gggtcttcta
      481 cctgaagatg aagggtgact actaccgcta cctggccgag gtggccaccg gtgacgacaa
      541 gaagcgcatc attgactcag cccggtcagc ctaccaggag gccatggaca tcagcaagaa
      601 ggagatgccg cccaccaacc ccatccgcct gggcctggcc ctgaactttt ccgtcttcca
      661 ctacgagatc gccaacagcc ccgaggaggc catctctctg gccaagacca ctttcgacga
      721 ggccatggct gatctgcaca ccctcagcga ggactcctac aaagacagca ccctcatcat
      781 gcagctgctg cg
//
