LOCUS       sequence-18526-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Wilker EW, Grant RA, Artim SC, Yaffe MB
  TITLE     A structural basis for 14-3-3sigma functional specificity.
  JOURNAL   J Biol Chem. 2005 May 13. 280(19):18891-8.
  PUBMED    15731107
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 May 13. 280(19):18891-8."
COMMENT     Sequence Label: pcDNA3 HA 14-3-3 sigma
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(783..809)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(789..815)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(789..815)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(837..856)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(838..856)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gaatagggcc ctctagatgc atgctcgagt cagctctggg gctcctgggg agcctcgccc
       61 ccctcttccc cggcgttgtc ggccgtccac agtgtcaggt tgtctcgcag cagctgcatg
      121 atgagggtgc tgtctttgta ggagtcctcg ctgagggtgt gcagatcagc catggcctcg
      181 tcgaaagtgg tcttggccag agagatggcc tcctcggggc tgttggcgat ctcgtagtgg
      241 aagacggaaa agttcagggc caggcccagg cggatggggt tggtgggcgg catctccttc
      301 ttgctgatgt ccatggcctc ctggtaggct gaccgggctg agtcaatgat gcgcttcttg
      361 tcgtcaccgg tggccacctc ggccaggtag cggtagtagt cacccttcat cttcaggtag
      421 aagacccggc tctcggcgtc cccggcctcc ttgatgaggt ggctgtccag caggcccagc
      481 acggtgtcgc acacgccctg gagctcagtc tccaccttct cccggtactc acgcacctcg
      541 ggccccttct cctccgagcc ctcctcgttg cttttctgct caatactgga cagcaccctc
      601 caggcagccc tctggccgcc caccacgttc ttataggcta ctgagagcag gtttcgctct
      661 tcgcaggaga gctcctcgcc cttctccacg gcgcctttca tgaaggctgc catgtcctca
      721 tagcgttcgg cctgctctgc cagcttggcc ttctggatca gactggctct ctccatggat
      781 ccgagggagg cgtagtcggg cacgtcgtag gggtaggcca tggtaagctt gggtctccct
      841 atagtgagtc gtattaattt cgataagcca gtaagcagtg ggttctctag ttagccagag
      901 agctctgctt atatag
//
