LOCUS       sequence-18527-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Cole NB, Smith CL, Sciaky N, Terasaki M, Edidin M, 
            Lippincott-Schwartz J
  TITLE     Diffusional mobility of Golgi proteins in membranes of living cells.
  JOURNAL   Science. 1996 Aug 9. 273(5276):797-801.
  PUBMED    8670420
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1996 Aug 9. 273(5276):797-801."
COMMENT     Sequence Label: ECFP-GalT
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      265..274
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(271..273,274..276,277..789)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(316..337)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(577..596)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccgtcagatc cgctagcgct accggactca gatctcgagc tcgccaccat gaggcttcgg
       61 gagccgctcc tgagcggcag cgccgcgatg ccaggcgcgt ccctacagcg ggcctgccgc
      121 ctgctcgtgg ccgtctgcgc tctgcacctt ggcgtcaccc tcgtttacta cctggctggc
      181 cgcgacctga gccgcctgcc ccaactggtc ggagtctcca caccgctgca gtcgacggta
      241 ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
      301 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacag gttcagcgtg
      361 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      421 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag
      481 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      541 gaaggctacg tccaggagcg taccatcttc ttcaaggacg acggcaacta caagacccgc
      601 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      661 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacat cagccacaac
      721 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg cccacttcaa gatccgccac
      781 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
      841 gacggccccg tgctgctgcc cgacaaccac tacctgagca ccagtccgcc ctgagcaaag
      901 acccca
//
