LOCUS       sequence-18529-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Cole NB, Smith CL, Sciaky N, Terasaki M, Edidin M, 
            Lippincott-Schwartz J
  TITLE     Diffusional mobility of Golgi proteins in membranes of living cells.
  JOURNAL   Science. 1996 Aug 9. 273(5276):797-801.
  PUBMED    8670420
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1996 Aug 9. 273(5276):797-801."
COMMENT     Sequence Label: Cerulean-GalT
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      268..277
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(274..276,277..279,280..792)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(319..340)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(580..599)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgaccgtcag atccgctagc gctaccggac tcagatctcg agctcgccac catgaggctt
       61 cgggagccgc tcctgagcgg cagcgccgcg atgccaggcg cgtccctaca gcgggcctgc
      121 cgcctgctcg tggccgtctg cgctctgcac cttggcgtca ccctcgttta ctacctggct
      181 ggccgcgacc tgagccgcct gccccaactg gtcggagtct ccacaccgct gcagtcgacg
      241 gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc
      301 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      361 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      421 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg
      481 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      541 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      601 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      661 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacgc catcagcgac
      721 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
      781 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagca
//
