LOCUS       sequence-18531-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Hoover CL, Hilgenberg LG, Smith MA
  TITLE     The COOH-terminal domain of agrin signals via a synaptic receptor in
            central nervous system neurons.
  JOURNAL   J Cell Biol. 2003 Jun 9. 161(5):923-32.
  PUBMED    12796478
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2003 Jun 9. 161(5):923-32."
COMMENT     Sequence Label: C-Ag20(8)
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..42)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(58..87)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(664..690)
                     /codon_start=1
                     /product="synthetic cistron containing a ribosome binding 
                     site (Shine-Dalgarno sequence), for enhancing the bacterial
                     expression of a downstream cistron (Schoner, 1997)"
                     /label=minicistron
                     /note="This first cistron should terminate 3 bp upstream of
                     the ATG for the second cistron."
                     /translation="MYRLNKEE"
ORIGIN
        1 ccagctggag accgtttaaa ctcaatgatg atgatgatga tggtcgacgg cgctattcag
       61 atcctcttct gagatgagtt tttgttcggg cccaagcttc gaattcagag tggggcaggg
      121 tcttagctct ggtttggtga cagcgtcctc cagcagatgc agctggcgat ggcccactac
      181 cacgtcccgc agacagccca caaaacccgt gccataggcc ttggggagag cctgccccac
      241 aggaagcttc tgtaggcctc caagccacag ggctccatct gtgtccaatt gtgtggcacc
      301 cagcggggaa gagccagtca caggggcttc attgcccacc tgaagggaac cttccctgtg
      361 ctccctgtga gccctgactc gaagccagcg gttggtgttg accttcacag tggagcgcag
      421 cacaactggc tgggagccta ggtcatagct cagttgtagg tgcccatcca caatggccag
      481 agccatgtag tctgcacgtt ctccaacctt tccaatccac agcaccagcc cctgcgtggc
      541 ctcagtgcgt aagctcagct caaagtggtt gctctgcagc gctttctcag ctgggatctc
      601 attggtcagc tcgctctcag tcacagcatt gaggtactcg atgtaggtcc gaggatccat
      661 ggtttattcc tccttattta atcgatacat taatatatac ctctttaatt tttaataata
      721 aagttaatcg ataa
//
