LOCUS       sequence-18539-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Patterson GH, Lippincott-Schwartz J
  TITLE     A photoactivatable GFP for selective photolabeling of proteins and 
            cells.
  JOURNAL   Science. 2002 Sep 13. 297(5588):1873-7.
  PUBMED    12228718
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2002 Sep 13. 297(5588):1873-7."
COMMENT     Sequence Label: pRSETA-PAGFP
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..31
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             join(47..49,50..52,53..565)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(92..113)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(353..372)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     700..721
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gnnncnngnn ctgtacgacg atgacgataa ggatcgatgg ggatccatgg tgagcaaggg
       61 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      121 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      181 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
      241 cagctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
      301 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      361 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      421 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      481 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggccaa
      541 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
      601 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagccacca
      661 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
      721 gaccgccgcc cgggatcact ctcggcatgg acgagctgta caagtaanaa ttcgaagctt
      781 gatccggctg ctaacaaagc ccnanangaa nnnnnannnn tngnnnngcn nnncaccg
//
