LOCUS       sequence-18544-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Brunet A, Kanai F, Stehn J, Xu J, Sarbassova D, Frangioni JV, Dalal 
            SN, DeCaprio JA, Greenberg ME, Yaffe MB
  TITLE     14-3-3 transits to the nucleus and participates in dynamic 
            nucleocytoplasmic transport.
  JOURNAL   J Cell Biol. 2002 Mar 4. 156(5):817-28.
  PUBMED    11864996
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2002 Mar 4. 156(5):817-28."
COMMENT     Sequence Label: pcDNA3.1/HisC 14-3-3 epsilon K49E
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..27
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..63
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(67..75,76..90)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(928..945)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gggggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg
       61 ggtcgggatc tgtacgacga tgacgataag gtaccaggat ccaccatgga tgatcgggag
      121 gatctggtgt accaggcgaa gctggcagag caggccgagc gatacgacga aatggtggaa
      181 tcaatgaaga aagtagcagg aatggacgtg gagctgacag ttgaagaacg aaacctttta
      241 tctgttgcat atgaaaatgt gattggagcc agaagagcat cctggagaat aatcagcagc
      301 attgaacaga aggaagaaaa caagggagga gaggacaaat taaagatgat tcgggagtac
      361 cggcaaatgg ttgaaactga gctcaagtta atctgttgtg acattctgga tgtactggac
      421 aagcacctca ttccagcagc taacactggc gagtccaagg ttttctatta taaaatgaaa
      481 ggggactacc acaggtatct ggctgagttt gccacaggaa acgacaggaa ggaggcagca
      541 gagaacagcc ttgtggctta caaagctgct agtgacattg cgatgacaga acttcctcca
      601 acgcacccca ttcgtttagg tcttgctctc aacttttccg tattctacta tgaaattctt
      661 aattcccccg accgtgcctg caggttggca aaagcagctt ttgatgacgc aattgcagaa
      721 ctggacacgc tgagtgaaga aagctataag gactccacac tcatcatgca gctgctacgt
      781 gataacctga cgctgtggac ctcagacatg cagggcgatg gtgaagagca gaataaagaa
      841 gcgctgcagg atgtggaaga tgagaatcag tgatctagag ttcgggtcga ctcgagtcta
      901 gagggcccgt ttnaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
      961 ttgtttgccc ctccccgtgc cttcctgacc
//
