LOCUS       sequence-18548-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Dantuma NP, Groothuis TA, Salomons FA, Neefjes J
  TITLE     A dynamic ubiquitin equilibrium couples proteasomal activity to 
            chromatin remodeling.
  JOURNAL   J Cell Biol. 2006 Apr 10. 173(1):19-26.
  PUBMED    16606690
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2006 Apr 10. 173(1):19-26."
COMMENT     Sequence Label: GFP-Ub KO.G76V
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..268
                     /codon_start=1
                     /product="human ubiquitin variant in which all of the 
                     lysines have been mutated to arginine"
                     /label=ubiquitin-K0
                     /note="also known as ubiquitin-KO"
                     /translation="MQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIF
                     AGRQLEDGRTLSDYNIQRESTLHLVLRLRG"
     polyA_signal    419..540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(456..475)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     510..529
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(684..705)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     896..915
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgtacaagtc cggactcaga tctcgagctc agcttatata catatgcaga tcttcgtcag
       61 aacgttaacc ggtagaacca taactctaga agttgaacca tccgatacca tcgaaaacgt
      121 tagagctaga attcaagaca gagaaggcat tccacctgat caacaaagat tgatctttgc
      181 cggtagacag ctcgaggacg gtagaacgct gtctgattac aacattcaga gagagtcgac
      241 cttacatctt gtcttaagac taagaggtgt gtgaggtacc gcgggcccgg gatccaccgg
      301 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
      361 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
      421 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
      481 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
      541 acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
      601 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      661 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      721 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      781 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      841 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      901 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgt
//
