LOCUS       sequence-18549-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Dantuma NP, Groothuis TA, Salomons FA, Neefjes J
  TITLE     A dynamic ubiquitin equilibrium couples proteasomal activity to 
            chromatin remodeling.
  JOURNAL   J Cell Biol. 2006 Apr 10. 173(1):19-26.
  PUBMED    16606690
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2006 Apr 10. 173(1):19-26."
COMMENT     Sequence Label: PAGFP-Ub
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..265
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     polyA_signal    413..534
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(450..469)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     504..523
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(678..699)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agtccggact cagatctcga gctcagcttc gggccgcatg cagatcttcg tgaagactct
       61 gactggtaag accatcaccc tcgaggttga gcccagtgac accattgaga atgtcaaggc
      121 aaagatccaa gataaggaag gcatccctcc tgaccagcag aggctgatct ttgctggaaa
      181 acagctggaa gatgggcgca ccctgtctga ctacaacatc cagaaagagt ccaccctgca
      241 cctggtactc cgtctcagag gtgggtgagg taccgcgggc ccgggatcca ccggatctag
      301 ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
      361 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
      421 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
      481 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt
      541 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
      601 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      661 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      721 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      781 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaaggg
//
