LOCUS       sequence-185521-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human GRK5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: GRK5 gRNA (BRDN0001149127)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        151..226
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    282..399
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
       61 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
      121 acgaaacacc gggcctgagc cccgactact gttttagagc tagaaatagc aagttaaaat
      181 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttaagcttgg
      241 cgtaactaga tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
      301 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
      361 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
      421 agagatccac tttggcgccg gctcgagggg gcccgggtgc aaagatggat aaagttttaa
      481 acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc
      541 tccggtgccc gtcagtgggc agagcgcaca
//
