LOCUS       sequence-18556-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Menendez-Benito V, Verhoef LG, Masucci MG, Dantuma NP
  TITLE     Endoplasmic reticulum stress compromises the ubiquitin-proteasome 
            system.
  JOURNAL   Hum Mol Genet. 2005 Oct 1. 14(19):2787-99.
  PUBMED    16103128
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2005 Oct 1. 14(19):2787-99."
COMMENT     Sequence Label: Ub-G76V-YFP
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..286
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRG"
     regulatory      323..332
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(329..331,332..334,335..793)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(374..395)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(635..654)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgaaccgtag atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattcac
       61 catgcagatc ttcgtgaaga ctctgactgg taagaccatc accctcgagg ttgagcccag
      121 tgacaccatt gagaatgtca aggcaaagat ccaagataag gaaggcatcc ctcctgacca
      181 gcagaggctg atctttgctg gaaaacagct ggaagatggg cgcaccctgt ctgactacaa
      241 catccagaaa gagtccaccc tgcacctggt actccgtctc agaggtgtgg tggggaagct
      301 tggtcgacag gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
      361 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      421 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      481 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcgtgcagtg
      541 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      601 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      661 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      721 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
      781 ctatatcatg gccgacaagc agaggaacgg catcaaggtg aacttcaaga tccgccac
//
