LOCUS       sequence-18559-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Heessen S, Dantuma NP, Tessarz P, Jellne M, Masucci MG
  TITLE     Inhibition of ubiquitin/proteasome-dependent proteolysis in 
            Saccharomyces cerevisiae by a Gly-Ala repeat.
  JOURNAL   FEBS Lett. 2003 Dec 4. 555(2):397-404.
  PUBMED    14644450
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2003 Dec 4. 555(2):397-404."
COMMENT     Sequence Label: pYES2-Ub-M-GFP
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..322
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     regulatory      356..365
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(407..428)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(668..687)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 actagcagct gtatacgact cactataggg aatattaagc ttggtaccga gctcggatcc
       61 actagtaacg gccgccagtg tgctggaatt caccatgcag atcttcgtga agactctgac
      121 tggtaagacc atcaccctcg aggttgagcc cagtgacacc attgagaatg tcaaggcaaa
      181 gatccaagat aaggaaggca tccctcctga ccagcagagg ctgatctttg ctggaaaaca
      241 gctggaagat gggcgcaccc tgtctgacta caacatccag aaagagtcca ccctgcacct
      301 ggtactccgt ctcagaggtg ggatggggaa gcttggtcga caggatccac cggtcgccac
      361 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      421 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      481 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      541 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
      601 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      661 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      721 ggtgaaccgc atcgagctga agggcat
//
