LOCUS       sequence-18560-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Heessen S, Dantuma NP, Tessarz P, Jellne M, Masucci MG
  TITLE     Inhibition of ubiquitin/proteasome-dependent proteolysis in 
            Saccharomyces cerevisiae by a Gly-Ala repeat.
  JOURNAL   FEBS Lett. 2003 Dec 4. 555(2):397-404.
  PUBMED    14644450
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2003 Dec 4. 555(2):397-404."
COMMENT     Sequence Label: pYES2-Ub-R-GFP
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             89..316
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     regulatory      350..359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(401..422)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agctgtatac gactcactat agggaatatt aagcttggta ccgagctcgg atccactagt
       61 aacggccgcc agtgtgctgg aattcaccat gcagatcttc gtgaagactc tgactggtaa
      121 gaccatcacc ctcgaggttg agcccagtga caccattgag aatgtcaagg caaagatcca
      181 agataaggaa ggcatccctc ctgaccagca gaggctgatc tttgctggaa aacagctgga
      241 agatgggcgc accctgtctg actacaacat ccagaaagag tccaccctgc acctggtact
      301 ccgtctcaga ggtgggcgcg ggaagcttgg tcgacaggat ccaccggtcg ccaccatggt
      361 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      421 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      481 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      541 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
      601 cgacttcttc aagtccgcca tgcccgaagg ctgcgtccag cagcgcccca tcttcttcta
      661 ggacgacggc gactactaga cccgctccga ggaggagttc tagggcgac
//
