LOCUS       sequence-18563-e         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter gene with five ATF6 sites that is highly sensitive to ER 
            stress.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Wang Y, Shen J, Arenzana N, Tirasophon W, Kaufman RJ, Prywes R
  TITLE     Activation of ATF6 and an ATF6 DNA binding site by the endoplasmic 
            reticulum stress response.
  JOURNAL   J Biol Chem. 2000 Sep 1. 275(35):27013-20.
  PUBMED    10856300
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Sep 1. 275(35):27013-20."
COMMENT     Sequence Label: p5xATF6-GL3
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    378..469
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(410..429)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    483..531
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cgccgggcct ttctttatgt ttttggcgtc ttccatggag atcctctaga gtcgactcta
       61 gaggccgccg gctcagtctt ggcttctcag ttgctcgctg cagatgcggt tggagtacga
      121 ggcgccgcag ccactgcttt tataacaagc gttttatgaa tgagtgcaga tctcgaggaa
      181 tcgccacgtc agcacctgtc tcgaggaatc gccacgtcag cacctgtctc gaggaatcgc
      241 cacgtcagca cctgtctcga ggaatcgcca cgtcagcacc tgtctcgagg aatcgccacg
      301 tcagcacctg tctcgagcaa gcttgggctg caggtcgagc ccgggctagc acgcgtaaga
      361 gctcggtacc tatcgataga gaaatgttct ggcacctgca cttgcactgg ggacagccta
      421 ttttgctagt ttgttttgtt tcgttttgtt ttgatggaga gcgtatgtta gtactatcga
      481 ttcacacaaa aaaccaacac acagatgtaa tgaaaataaa gatattttat tgcggccgct
      541 ccaagtacct cccgtacctt aatattactt acttatcatg gtagcttggg ctggcgtaat
      601 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      661 caaattgtaa gcgttaatat tttgttaaaa ttcgcg
//
