LOCUS       sequence-18565-e         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of human MKL1 with a 3x FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Cen B, Selvaraj A, Burgess RC, Hitzler JK, Ma Z, Morris SW, Prywes R
  TITLE     Megakaryoblastic leukemia 1, a potent transcriptional coactivator 
            for serum response factor (SRF), is required for serum induction of 
            SRF target genes.
  JOURNAL   Mol Cell Biol. 2003 Sep . 23(18):6597-608.
  PUBMED    12944485
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2003 Sep . 23(18):6597-608."
COMMENT     Sequence Label: p3xFLAG-MKL1
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..90
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(445..456)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aagtgaaccg tcaaattaac catggactac aaagaccatg acggtgatta taaagatcat
       61 gacatcgatt acaaggatga cgatgacaag cttgcggccg cgaattcgat gccgcctttg
      121 aaaagtccag ccgcatttca tgagcagaga aggagcttgg agcgggccag gacagaggac
      181 tatctcaaac ggaagattcg ttcccggccg gagagatcgg agctggtcag gatgcacatt
      241 ttggaagaga cctcggctga gccatccctc caggccaagc agctgaagct gaagagagcc
      301 agactagccg atgacctcaa tgagaagatt gcacagaggc ctggccccat ggagctggtg
      361 gagaagaaca tccttcctgt tgagtccagc ctgaaggaag ccatcattgt gggccaggtg
      421 aactatccca aagtagcaga cagctcttcc ttcgatgagg acagcagcga tgccttatcc
      481 cccgagcagc ctgccagcca tgagtcccag ggttctgtgc cgtcacccct ggaggcccga
      541 gtcagcgaac cactgctcag tgccacctct gcatccccca cccaggttgt gtctcaactt
      601 ccgatgggcc gggat
//
