LOCUS       sequence-18571-e         388 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Kissler S, Stern P, Takahashi K, Hunter K, Peterson LB, Wicker LS
  TITLE     In vivo RNA interference demonstrates a role for Nramp1 in modifying
            susceptibility to type 1 diabetes.
  JOURNAL   Nat Genet. 2006 Apr . 38(4):479-83.
  PUBMED    16550170
REFERENCE   2  (bases 1 to 388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2006 Apr . 38(4):479-83."
COMMENT     Sequence Label: pLB-915
FEATURES             Location/Qualifiers
     source          1..388
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     96..112
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     97..113
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(155..188)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tgttttgaga ctataaatat cccttggaga aaagccttgt ttgccacatg tacttcctga
       61 ttcaagagat caggaagtac atgttggctt ttttctcgag gtcgacggta tcgataagct
      121 cgcttcacga gattccagca ggtcgaggga cctaataact tcgtatagca tacattatac
      181 gaagttatat taagggttcc aagcttaagc ggccgcgtgg ataaccgtat taccgccatg
      241 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      301 ccgcgttaca taacttacgg taaatggccc gcctggctga ccggccatcg acccccgccc
      361 attgacgtca ataatgacgt atgttccc
//
