LOCUS       sequence-18572-e         598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 598)
  TITLE     Johnson Lab Plasmids
REFERENCE   2  (bases 1 to 598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-HA-CIRBP
FEATURES             Location/Qualifiers
     source          1..598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             97..123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      136..145
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttgccgcc accatgtacc cttatgatgt gccagattat
      121 gcctctgaat tcgccgccac catggcatca gatgaaggca aactttttgt tggagggctg
      181 agttttgaca ccaatgagca gtcgctggag caggtcttct caaagtacgg acagatctct
      241 gaagtggtgg ttgtgaaaga cagggagacc cagagatctc ggggatttgg gtttgtcacc
      301 tttgagaaca ttgacgacgc taaggatgcc atgatggcca tgaatgggaa gtctgtagat
      361 ggacggcaga tccgagtaga ccaggcaggc aagtcgtcag acaaccgatc ccgtgggtac
      421 cgtggtggct ctgccggggg ccggggcttc ttccgtgggg gccgaggacg gggccgtgga
      481 ttctctagag gaggagggga ccgaggctat ggggggaacc ggttcgagtc caggagtggg
      541 ggctacggag gctccagaga ctgctatagc agccggagtc agagtggtgg ctacagtg
//
