LOCUS       sequence-18574-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  TITLE     Johnson Lab Plasmids
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-CCL21-V5
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..68)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      488..497
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(567..586)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(568..586)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(632..684)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
ORIGIN
        1 tggtgatggt gatgatgacc ggtacgcgta gaatcgagac cgaggagagg gttagggata
       61 ggcttacctt cgaatgggtg acctcgagtt ggccctttag gggtctgtga ccgctcagtc
      121 ctcttgcagc ctttggagcc ctttcctttc ttgccagtct tggaggcccc cctgtccttc
      181 ctgcagccct gggctggttt ctgtggggat ggtgtcttgt ccagatgctg catcagctgc
      241 tgcacccaga gctcctttgg gtctgcacat agctctgcct gagagcgctt gcggggcaag
      301 aacaggatag ctgggatgga gcagcctaag cttggttcct gcttccggta gctgcggaca
      361 accttggcgg gaatcttcct ttggctgtac ttgaggcaac agtcctgagc ccctccatca
      421 ctgccttggg tcctggggat gccaaaggcc agaaccagga taaggaggct cagagccagt
      481 gactgagcca tggtggcggc gaattccacc acactggact agtggatccg agctcggtac
      541 caagcttaac tagccagctt gggtctccct atagtgagtc gtattaattt cgataagcca
      601 gtaagcagtg ggttctctag tttagccaga gagctctgct tatatagacc tcccaccgta
      661 cacgccttac cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc
      721 cgttgatttt ggtgccaaac aaactcccat tgacgtcaat
//
