LOCUS       sequence-18575-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  TITLE     Johnson Lab Plasmids
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-HA-ADH
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             93..119
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt gccgccacca tgtaccctta tgatgtgcca gattatgcct
      121 ctgaattcat gtcatcctca ggcacgccag acttacctgt cctactcacc gatttgaaga
      181 ttcaatatac taagatcttc ataaacaatg aatggcatga ttcagtgagt ggcaagaaat
      241 ttcctgtctt taatcctgca actgaggagg agctctgcca ggtagaagaa ggagataagg
      301 aggatgttga caaggcagtg aaggccgcaa gacaggcttt tcagattgga tccccgtggc
      361 gtactatgga tgcttccgag agggggcgac tattatacaa gttggctgat ttaatcgaaa
      421 gagatcgtct gctgctggcg acaatggagt caatgaatgg tggaaaactc tattccaatg
      481 catatctgaa tgatttagca ggctgcatca aaacattgcg ctactgtgca ggttgggctg
      541 acaagatcca gggccgtaca ataccaattg atggaaattt ttttacatat acaagacatg
      601 aacctattgg tgtatgtggc caaatcattc cttggaattt cccgttggtt atgctcattt
      661 ggaagatagg gcctgcactg agctgtggaa acacagtggt tgtcaaacca gcagagcaaa
      721 ctcctctcac tgctctccac gtggcatctt
//
