LOCUS       sequence-18579-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  TITLE     Johnson Lab Plasmids
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-HA-p48
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             328..336
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cncanncgcn canntccctt atgatgtgcc agattatgcc tctgaattcg catcaggcag
       61 ggcacgctgc acccgaaaac tccggaactg ggtggtggag caagtggaga gtgggcagtt
      121 tcccggagtg tgctgggatg atacagctaa gaccatgttc cggattccct ggaaacatgc
      181 aggcaagcag gacttccggg aggaccagga tgctgccttc ttcaaggcct gggcaatatt
      241 taagggaaag tataaggagg gggacacagg aggtccagct gtctggaaga ctcgcctgcg
      301 ctgtgcactc aacaagagtt ctgaatttaa ggaggttcct gagaggggcc gcatggatgt
      361 tgctgagccc tacaaggtgt atcagttgct gccaccagga atcgtctctg gccagccagg
      421 gactcagaaa gtaccatcaa agcgacagca cagttctgtg tcctctgaga ggaaggagga
      481 agaggatgcc atgcagaact gcacactcag tccctctgtg ctccaggact ccctcaataa
      541 tgaggaggag ggggccagtg ggggagcagt ccattcagac attgggagca gcagcagcag
      601 cagcagccct gagccacagg aagttacaga cacaactgan cccccctttc agggggatca
      661 gaggtcctgg agtttctngt tcctccagag ccagactact cactgctgct caccttcatc
      721 tacaacgggc gcgtggtggg cgagcccagg tncaaagcct ggattgccgc cttgtggctg
      781 accctcagct ctgaaanaca tgtnaaangg tgttttccca ngctggccnt tgnacccacc
      841 aacccctntg atcaactt
//
