LOCUS       sequence-185799-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human IRAK1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: IRAK1 gRNA (BRDN0001145800)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        73..148
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    204..321
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cgaggagtac
       61 atcaagacgg gagttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
      121 acttgaaaaa gtggcaccga gtcggtgctt ttttaagctt ggcgtaacta gatcttgaga
      181 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
      241 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
      301 attacaaaaa ttcaaaattt tcgggtttat tacaggggac agcagagatc cactttggcg
      361 ccggctcgag ggggcccggg tgcaaagatg gataaagttt taaacagaga ggaatctttg
      421 cagctaatgg accttctagg tcttgaaagg gagngggaat tggctccggt gcccgncagt
      481 gggcagagcg cacattcgcc cacagncccc gagaagttgg gggggagggg tccgcaaatt
      541 gatccgggtg cctanagaag gtggcgcggg
//
