LOCUS       sequence-18583-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  TITLE     Johnson Lab Plasmids
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-GFP-p48
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(72..91)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(73..91)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(136..339)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(165..185)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        340..643
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cnccggggac gctcctcgcc cttgctagcc atttcttgcg cgcccgcgga ggctggatca
       61 agcttgggtc tccctatagt gagtcgtatt aatttcgata agccagtaag cagtgggttc
      121 tctagttagc cagagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
      181 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
      241 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
      301 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
      361 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
      421 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
      481 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
      541 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
      601 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcgaagac nnnnnngggc
      661 tataanctac gaccccgtan ttgattacta tnaataacta gtcaataatc antgtcacgc
      721 gtatatctgg ccgtacatcg cgancagcgc aaacgcctaa cctancagat cttcngcatt
      781 gtcgncagcc aggctgtgac taattgtcng atatcacgcn caccaacagg cgaannattg
      841 tnatngg
//
