LOCUS       sequence-18588-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-regulated lentiviral shRNA vector. Cre addition causes the 
            puromycin gene to be recombined out of the construct, activating 
            shRNA expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L,
            Jaenisch R, Jacks T
  TITLE     Cre-lox-regulated conditional RNA interference from transgenes.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5.
  PUBMED    15240889
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jul 13. 101(28):10380-5."
COMMENT     Sequence Label: pSico PGK puro
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(44..63)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     437..460
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    537..570
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     complement(588..608)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 ggcccggaga tgaggaagag gagaacagcg cggcagacgt gcgcttttga agcgtgcaga
       61 atgccgggcc tccggaggac cttcgggcgc ccgccccgcc cctgagcccg cccctgagcc
      121 cgcccccgga cccacccctt cccagcctct gagcccagaa agcgaaggag caaagctgct
      181 attggccgct gccccaaagg cctacccgct tccattgctc agcggtgctg tccatctgca
      241 cgagactagt gagacgtgct acttccattt gtcacgtcct gcacgacgcg agctgcgggg
      301 cgggggggaa cttcctgact aggggaggag tagaaggtgg cgcgaagggg ccaccaaaga
      361 acggagccgg ttggcgccta ccggtggatg tggaatgtgt gcgaggccag aggccacttg
      421 tgtagcgcca agtgcccagc ggggctgcta aagcgcatgc tccagactgc cttgggaaaa
      481 gcgcctcccc tacccggtag aatttcgagg tcgacgaatt caaaaaacca aggcttataa
      541 cttcgtataa tttatactat acgaagttat aattacttta cagttagggt gagtttcctt
      601 ttgtgctgtt ttttaaaata ataatttagt atttgtatct cttatagaaa tccaagccta
      661 tcatgtaaaa tgtagctagt attaaaaaga acagattatc tgtcttttat cgcacattaa
      721 gcctctatag ttactaggaa atattatatg caaattaacc ggggcagggg agtag
//
