LOCUS       sequence-186009-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human EPHA8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: EPHA8 gRNA (BRDN0001146924)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        155..230
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
       61 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
      121 aggacgaaac accgcctcaa aatcgacacc attggtttta gagctagaaa tagcaagtta
      181 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttaagc
      241 ttggcgtaac tagatcttga gacaaatggn cagtattcat ccncaatttt aaaagaaaag
      301 ggggggattg ggggggtaca gtgcagggga aaagaaatag tagacntaat agccaccgac
      361 cttccaactt aaagaatttc caaaaccaat ttccaaaant tncnaaattt tccggggttt
      421 nttnacnggg gncngncnaa aaatcccccn tttgggcccc ccgcccccan gggggccccg
      481 ggggcnaaaa aangggaaaa aggtttttnn aaccaaannn ggaaaccctt tgncccccca
      541 annggggncc tntcnnaggc cctngaaaag
//
