LOCUS       sequence-186069-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human ADCK4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: ADCK4 gRNA (BRDN0001145019)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        88..163
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    219..336
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
       61 aacaccggga gtgtgactac cgtcgtggtt ttagagctag aaatagcaag ttaaaataag
      121 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctttttta agcttggcgt
      181 aactagatct tgagacaaat ggnagtattc atccacaatt ttaaaagaaa aggggggatt
      241 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactana
      301 gaattacaaa aacaaattac aaaaattcaa aattttcngg tttattacag ggacagcaga
      361 gatccacttt ggcnccggct cgagggggcc cgggtgcaaa gatggataaa gtttttaaac
      421 agagagggat cttttgcagc ntaatggacc ttcnaggtct tgaaaggagt ggggaattgg
      481 ctccggtgcc cgtccagttg ggcagagcgc acatcgcccc acagtncccc gnagaagttg
      541 gggngngggg gcgggcaatt tganccggtg
//
