LOCUS       sequence-186155-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human DGKA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: DGKA gRNA (BRDN0001147297)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     82..101
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tattagtacc ccntttnngg ggncgtagaa agtaatattt tcgntgggta gtttgccctt
       61 ttaaaantat gtttnannan ggactatcat atgcttaccg taacttgaaa gtatttcgat
      121 ttcntggctt tatatatctg gnggaaagga cgaaacaccg ctcccaaact gacctctagg
      181 gtnttagagc tagaaatagc aagttaaant naggctagtc cgttatcaac ttgaaaaagt
      241 ggcaccgagg cggtgctttt ttangctngg cgnaactaga tcttgagacn aanggcanta
      301 ttcanccnca attttaaaag aaaagggggg atnggggggt acagngcagg ggaaagaata
      361 ntanacttaa nagcaacnga catacaaact aaagaattac aaanacnaat tacaaaaatt
      421 cnnaattttc gggtttatna cngggacagt antatatccn ctttggcgcc ggctcgaggg
      481 ggcccgggtg caaagatgta taaagtttta aacananagg aatctttgcn gctaatggac
      541 cttnctangn tcnatgaaan gagggtnaat
//
