LOCUS       sequence-186203-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human PRKCZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: PRKCZ gRNA (BRDN0001149519)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        147..222
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    278..395
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
       61 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      121 acaccgcctg cttccagacg acaagtgttt tagagctaga aatagcaagt taaaataagg
      181 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttaa gcttggcgta
      241 actagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
      301 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
      361 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
      421 atccactttg gcgccggctc gagggggccc gggtgcaaag atggataaag ttttaaacag
      481 agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg
      541 gtgcccgtca gtgggcagag cgcacatcgc
//
