LOCUS       sequence-186205-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human PRKCZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: PRKCZ gRNA (BRDN0001145480)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        144..219
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    275..392
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
       61 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
      121 ccgccccacc cccaggttct acggttttag agctagaaat agcaagttaa aataaggcta
      181 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttaagct tggcgtaact
      241 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
      301 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
      361 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
      421 cactttggcg ccggctcgag ggggcccggg tgcaaagatg gataaagttt taaacagaga
      481 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
      541 cccgtcagtg ggcagagcgc acatcgccca
//
