LOCUS       sequence-186225-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human TGFBR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: TGFBR1 gRNA (BRDN0001146778)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        119..194
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    250..367
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aaaattatgt tttagaatgg actatcntat gcttaccgta acttgaaagt atttcgattt
       61 cttggcttta tatatcttgt ggaaaggacn aaacaccgag aacgttcgtg gttccgtggt
      121 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
      181 caccgagtcg gtgctttttt angcttggcg taactagatc ttgagacaaa tggcagtatt
      241 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
      301 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta cnaaaattca
      361 aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc tcgagggggc
      421 ccgggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
      481 ctaggtcttg aaaggagtgg gaattggctc cggtgcccgn cagtgggcag agcgcacatc
      541 gcccacagtc cccgagaagt tggggggagg
//
