LOCUS       sequence-186239-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human MAP2K4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: MAP2K4 gRNA (BRDN0001145410)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     74..93
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        173..248
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    304..421
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aantacggtg cacgctagca aagtaataat ttcttgggta gctttgcagc ttttaaaatt
       61 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
      121 tttatatatc ttgtggaaag gacgaaacac cgtttgtaaa acttatcaaa cggttttaga
      181 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
      241 gtcggtgctt ttttaagctt ggcgtaacta gatcttgaga caaatggcag tattcatcca
      301 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
      361 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
      421 tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg gggcccgggt
      481 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
      541 cttgaaagga gtgggaattg gctccggtgc
//
