LOCUS       sequence-186249-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human MAP3K5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: MAP3K5 gRNA (BRDN0001147988)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     33..52
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        132..207
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    263..380
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
       61 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc gctgacttcg
      121 gaacatcaaa ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
      181 cttgaaaaag tggcaccgag tcggtgcttt tttaagcttg gcgtaactag atcttgagac
      241 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
      301 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
      361 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
      421 ggctcgaggg ggcccgggtg caaagatgga taaagtttta aacagagagg aatctttgca
      481 gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg
      541 cagagcgcac atcgcccaca gtccccgaga
//
