LOCUS       sequence-186305-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human TIE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: TIE1 gRNA (BRDN0001147382)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        150..225
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    281..398
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
       61 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      121 gaaacaccga gacccactgt ggatagacgg ttttagagct agaaatagca agttaaaata
      181 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt taagcttggc
      241 gtaactagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
      301 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      361 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      421 gagatccact ttggcgccgg ctcgaggggg cccgggtgca aagatggata aagttttaaa
      481 cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct
      541 ccggtgcccg tcagtgggca gagcgcacat
//
