LOCUS       sequence-186307-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human TIE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: TIE1 gRNA (BRDN0001144893)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        151..226
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    282..399
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
       61 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      121 cgaaacaccg acgtgacgtt aatgaacctg gttttagagc tagaaatagc aagttaaaat
      181 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttaagcttgg
      241 cgtaactaga tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
      301 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
      361 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
      421 agagatccac tttggcgccg gctcgagggg gcccgggtgc aaagatggat aaagttttaa
      481 acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc
      541 tccggtgccc gtcagtgggc agagcgcaca
//
