LOCUS       sequence-186345-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human RAF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: RAF1 gRNA (BRDN0001148013)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..87
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        167..242
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    298..415
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtgcacgcta gcaaagctaa taatttcttg ggtagctttg cagcttttaa aattatgttt
       61 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      121 tatcttgtgg aaaggacgaa acaccggccg aacaagcaaa gaacaggttt tagagctaga
      181 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
      241 gcttttttaa gcttggcgta actagatctt gagacaaatg gcagtattca tccacaattt
      301 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
      361 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
      421 ttattacagg gacagcagag atccactttg gcgccggctc gagggggccc gggtgcaaag
      481 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
      541 aggagtggga attggctccg gtgcccgtca
//
