LOCUS       sequence-18668-e         667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to 
            be recombined out of the construct, activating p53 shRNA 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L,
            Jaenisch R, Jacks T
  TITLE     Cre-lox-regulated conditional RNA interference from transgenes.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5.
  PUBMED    15240889
REFERENCE   2  (bases 1 to 667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jul 13. 101(28):10380-5."
COMMENT     Sequence Label: pSico p53
FEATURES             Location/Qualifiers
     source          1..667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(21..54)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             complement(73..84)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             94..105
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     120..136
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     121..137
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(265..285)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 agctgtacag tagcggccgc ataacttcgt atagtataaa ttatacgaag ttataagcct
       61 tgtttgtact ctcctcccct caatttcaag agaattgagg ggaggagagt acttttttct
      121 cgaggtcgac ggtatcgata agctcgcttc acgagatcat gtttaagggt tccggttcca
      181 ctaggtacaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
      241 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
      301 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
      361 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
      421 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
      481 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
      541 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
      601 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt ggcacctgga ttctgcgcgg
      661 gacgtcc
//
