LOCUS       sequence-18673-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Jackson EL, Willis N, Mercer K, Bronson RT, Crowley D, Montoya R, 
            Jacks T, Tuveson DA
  TITLE     Analysis of lung tumor initiation and progression using conditional 
            expression of oncogenic K-ras.
  JOURNAL   Genes Dev. 2001 Dec 15. 15(24):3243-8.
  PUBMED    11751630
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2001 Dec 15. 15(24):3243-8."
COMMENT     Sequence Label: LSL K-ras G12D
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(361..380)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(362..380)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(390..407)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(390..406)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cctatnnccg cgncatgtta cancccttac aggcgtgggg gaggggagcg gtcgcgaact
       61 ccgcggcgga ttcctttccc ctccgagaac cacgacgccc tccggccacc gggtccgagc
      121 acctcccgcc gcgacctccc ccggggcccc gcagcccggc caggggaagc ggcgtccccg
      181 cacagcccgc gcgcccgaca agttgagccg ggcgcggggc gccaggnncg gatgccgggt
      241 gcagcagagg ccgggcgcgn aggccgggcg agcgcggagg gccggccgcg gccggnagga
      301 ggaaggaaag ccgccccgtc cgggaaacgg cggccgccac cgcggtggan ctccaattcg
      361 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcntgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcan cctgaatggc
      541 gaatgggacg cgccctgtag cggcgcatta agcgcggcng gtgtggtggt tacgcgcagc
      601 gtgaccgcta cacttgccag cgccntagcg nccgctcctt tngctttctt cccttccttt
      661 ctcgccacgt tcgccggctt ttcccccgtc aagtttttta atcngggggc tcccntttaa
      721 gggtttccna atttaagtgc tttnacgggc ancntcgaac ccaaaaaaan tttttc
//
