LOCUS       sequence-186911-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human RIPK3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: RIPK3 gRNA (BRDN0001147505)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        68..143
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    200..317
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgact gcttcgtaca
       61 cgagtgagtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
      121 aaaaagtggc accgagtcgg tgctttttta agcttggcgt aactagatct tgagacaaat
      181 ggcctgnatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
      241 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
      301 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
      361 tcgagggggc ccgggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
      421 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      481 agcgcacatc gcccacagnc cccgagaagt tggggggagg ggtcggcaat tgatccggtg
      541 cctaaagaag gtggcgcggg gtaaactggg
//
