LOCUS       sequence-18692-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Lebron JA, Bennett MJ, Vaughn DE, Chirino AJ, Snow PM, Mintier GA, 
            Feder JN, Bjorkman PJ
  TITLE     Crystal structure of the hemochromatosis protein HFE and 
            characterization of its interaction with transferrin receptor.
  JOURNAL   Cell. 1998 Apr 3. 93(1):111-23.
  PUBMED    9546397
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1998 
            Apr 3. 93(1):111-23."
COMMENT     Sequence Label: pAcGP67A-TfR
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     120..179
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             189..206
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             216..227
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(816..827)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttttgtataa aaaacctata aatattccgg attattcata ccgtcccacc atcgggcgcg
       61 gatctatgct actagtaaat cagtcacacc aaggcttcaa taaggaacac acaagcaaga
      121 tggtaagcgc tattgtttta tatgtgcttt tggcggcggc ggcgcattct gcctttgcgg
      181 cggatccgca tcatcatcat catcacagca gcggcatcga gggacgtggt gaattccgct
      241 tatattggga tgacctgaag agaaagttgt cggagaaact ggacagcaca gacttcacca
      301 gtaccatcaa gctgctgaat gaaaattcat atgtccctcg tgaggctgga tctcaaaaag
      361 atgaaaatct tgcgttgtat gttgaaaatc aatttcgtga atttaaactc agcaaagtct
      421 ggcgtgatca acattttgtt aagattcagg tcaaagacag cgctcaaaac tcggtgatca
      481 tagttgataa gaacggtaga cttgtttacc tggtggagaa tcctgggggt tatgtggcgt
      541 atagtaaggc tgcaacagtt actggtaaac tggtccatgc taattttggt actaaaaaag
      601 attttgagga tttatacact cctgtgaatg gatctatagt gattgtcaga gcagggaaaa
      661 tcacctttgc agaaaaggtt gcaaatgctg aaagcttaaa tgcaattggt gtgttgatat
      721 acatggacca gactaaattt cccattgtta acgcagaact ttcattcttt ggacatgctc
      781 atctggggac aggtgaccct tacacacctg gattcccttc cttcaatcac actcagtttc
      841 caccatctcg gtcatcagga ttgcctaata tacctgt
//
