LOCUS       sequence-18693-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Cai SL, Tee AR, Short JD, Bergeron JM, Kim J, Shen J, Guo R, Johnson
            CL, Kiguchi K, Walker CL
  TITLE     Activity of TSC2 is inhibited by AKT-mediated phosphorylation and 
            membrane partitioning.
  JOURNAL   J Cell Biol. 2006 Apr 24. 173(2):279-89.
  PUBMED    16636147
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2006 Apr 24. 173(2):279-89."
COMMENT     Sequence Label: Flag TSC2 2A(S939A + S981A)
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..122
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gaccgtcaga tccgctagcg attacgccaa gctcgaaatt aaccctcact aaagggaaca
       61 aaagctggag ctccaccgcg gtggcggccg ccaccatgga ttacaaggat gacgacgata
      121 agaagcccgg gcggatccca tcgaattccg gtgcgtcctg gtccaccatg gccaaaccaa
      181 caagcaaaga ttcaggcttg aaggagaagt ttaagattct gttgggactg ggaacaccga
      241 ggccaaatcc caggtctgca gagggtaaac agacggagtt tatcatcacc gcggaaatac
      301 tgagagaact gagcatggaa tgtggcctca acaatcgcat ccggatgata gggcagattt
      361 gtgaagtcgc aaaaaccaag aaatttgaag agcacgcagt ggaagcactc tggaaggcgg
      421 tcgcggatct gttgcagccg gagcggccgc tggaggcccg gcacgcggtg ctggctctgc
      481 tgaaggccat cgtgcagggg cagggcgagc gtttgggggt cctcagagcc ctcttcttta
      541 aggtcatcaa ggattaccct tccaacgaag accttcacga aaggctggag gttttcaagg
      601 ccctcacaga caatgggaga cacatcacct acttggagga agagctggct gactttgtcc
      661 tgcagtggat ggatgttggc ttgtcctcgg aattccttct ggtgctggtg aacttggtca
      721 aattcaatag ctgttacctc gacgagtaca tcgcaaggat ggttcagatg atctgtctgc
      781 tgt
//
