LOCUS       sequence-18697-e         647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Sherley JL
  TITLE     Guanine nucleotide biosynthesis is regulated by the cellular p53 
            concentration.
  JOURNAL   J Biol Chem. 1991 Dec 25. 266(36):24815-28.
  PUBMED    1761576
REFERENCE   2  (bases 1 to 647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1991 Dec 25. 266(36):24815-28."
COMMENT     Sequence Label: pLCR0
FEATURES             Location/Qualifiers
     source          1..647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(76..94)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        162..266
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(485..504)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcgagtgtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcg gatccgaatt
       61 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
      121 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      181 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      241 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      301 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      361 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      421 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      481 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
      541 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
      601 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatt
//
