LOCUS       sequence-187005-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human TSSK4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: TSSK4 gRNA (BRDN0001148876)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        149..224
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    280..397
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
       61 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
      121 aaacaccgta agaagggcta caacccacgt tttagagcta gaaatagcaa gttaaaataa
      181 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt aagcttggcg
      241 taactagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
      301 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
      361 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
      421 agatccactt tggcgccggc tcgagggggc ccgggtgcaa agatggataa agttttaaac
      481 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc
      541 cggtgcccgt cagtgggcag agcgcacatc
//
