LOCUS       sequence-18712-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Pertz O, Hodgson L, Klemke RL, Hahn KM
  TITLE     Spatiotemporal dynamics of RhoA activity in migrating cells.
  JOURNAL   Nature. 2006 Apr 20. 440(7087):1069-72.
  PUBMED    16547516
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Apr 20. 440(7087):1069-72."
COMMENT     Sequence Label: pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(334..585)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(376..397)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     725..744
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gttgggacag aaatgcttga cttctggggt ccacttttct gggatgtttt ctaaactatc
       61 agggctgtcg atggaaaaac acatcagtat aacatcggta tctgggtagg agaggggcct
      121 caggcgatca taatcttcct gcccagctgt gtcccacaaa gccaactcta cctgctttcc
      181 atccacctcg atatctgcca catagttctc aaacactgtg ggcacataca cctctgggaa
      241 ctggtccttg ctgaagacta tgagcaagca tgtctttcca caggctccat caccaacaat
      301 caccagtttc ttccggatgg cagccattcc ccccttgtac agctcgtcca tgccgagagt
      361 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
      421 tttgctcagg gcggactggt agctcaggta gtggttgtcg ggcagcagca cggggccgtc
      481 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt
      541 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac
      601 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
      661 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
      721 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
      781 gggcatggcg gact
//
