LOCUS       sequence-18713-e         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Pertz O, Hodgson L, Klemke RL, Hahn KM
  TITLE     Spatiotemporal dynamics of RhoA activity in migrating cells.
  JOURNAL   Nature. 2006 Apr 20. 440(7087):1069-72.
  PUBMED    16547516
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Apr 20. 440(7087):1069-72."
COMMENT     Sequence Label: pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(21..39,40..62,63..81,82..104,105..123,124..146,
                     147..165,166..188,189..207,208..230,231..249,250..272,
                     273..291)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        325..362
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     359..383
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             481..498
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgtcgtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
       61 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
      121 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
      181 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
      241 tgatagagaa aagtgaaagt cgagtttacc actccccatc cgtgatagag aaaagtgaaa
      301 gtcgagcttg gtaccccggt ccgagtaggc gtgtacggtg ggaggcctat ataagcagag
      361 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      421 gaagacaccg ggaccgatcc agcctccgcg gccccagatc cgtaaagtga attcatggca
      481 caccatcacc accatcacgg tagtggcatc ctggaggacc tcaatatgct ctacatccgg
      541 cagatggcac tcagcctgga ggacacagag ctgcagagga aactagatca tgagatccgg
      601 atgagggatg gggcctgcaa gctgctggca gcctgctccc agcgagagca ggctctggaa
      661 gccaccaaga gcctgctggt
//
