LOCUS       sequence-18714-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Ludwig TE, Bergendahl V, Levenstein ME, Yu J, Probasco MD, Thomson 
            JA
  TITLE     Feeder-independent culture of human embryonic stem cells.
  JOURNAL   Nat Methods. 2006 Aug . 3(8):637-646.
  PUBMED    16862139
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2006 Aug . 3(8):637-646."
COMMENT     Sequence Label: zbFGF
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             484..501
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(554..572)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      568..615
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(739..758)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggccaccgga gggatcacca cactcccgcc cgctccggac gccgaaaaca gcagctttcc
       61 cgcgggcagc ttcagggatc ccaagcgcct gtactgcaaa aacggaggat tcttcctgcg
      121 gatcaacgcg gacggccgag tggacggagc ccgagacaag aacgacccgc acattcgtct
      181 gcagctgcag gcgacggcag tgggtgaagt actcattaaa ggcatctgta ccaaccgttt
      241 ccttgccatg aacgcagacg gacgactgtt cgggacgaaa aggaccacag atgaatgtta
      301 tttcctggag cgcctggagt ccaacaacta caacacatac agatcccgca agtatcccga
      361 ctggtacgtg gctctgaaga gaaccggcca gtataaaagc ggctctaaaa ccagcccggg
      421 acagaaggcc atcctgtttc tgcccatgtc ggccaaatgc tgaagcttgc ggccgcactc
      481 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag
      541 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
      601 ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct
      661 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
      721 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
      781 gctttccccg tcaagctcta aatcgggggc tccct
//
