LOCUS       sequence-187155-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human TAF1L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: TAF1L gRNA (BRDN0001145988)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        145..220
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    276..393
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
       61 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
      121 accggaaggg caagtgtctc ctaggtttta gagctagaaa tagcaagtta aaataaggct
      181 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttaagc ttggcgtaac
      241 tagatcttga gacaaatggc agtattcntc cacaatttta aaagaaaagg ggggattggg
      301 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
      361 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
      421 ccactttggc gccggctcga gggggcccgg gtgcaaagat ggataaagtt ttaaacagan
      481 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccngt
      541 gcccgtcngt gggcagagcg cacatcgccc
//
