LOCUS       sequence-18716-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Wu CJ, Conze DB, Li T, Srinivasula SM, Ashwell JD
  TITLE     NEMO is a sensor of Lys 63-linked polyubiquitination and functions 
            in NF-kappaB activation.
  JOURNAL   Nat Cell Biol. 2006 Apr . 8(4):398-406.
  PUBMED    16547522
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2006 Apr . 8(4):398-406."
COMMENT     Sequence Label: pET-NEMO
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..84
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             88..120
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 ttgtttactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
       61 agcggcctgg tgccgcgcgg cagccatatg gctagcatga ctggtggaca gcaaatgggt
      121 cgcggatccg aattcaatag gcacctctgg aagagccaac tgtgtgagat ggtgcagccc
      181 agtggtggcc cggcagcaga tcaggacgta ctgggcgaag agtctcctct ggggaagcca
      241 gccatgctgc acctgccttc agaacagggc gctcctgaga ccctccagcg ctgcctggag
      301 gagaatcaag agctccgaga tgccatccgg cagagcaacc agattctgcg ggagcgctgc
      361 gaggagcttc tgcatttcca agccagccag agggaggaga aggagttcct catgtgcaag
      421 ttccaggagg ccaggaaact ggtggagaga ctcggcctgg agaagctcga tctgaagagg
      481 cagaaggagc aggctctgcg ggaggtggag cacctgaaga gatgccagca gcagatggct
      541 gaggacaagg cctctgtgaa agcccaggtg acgtccttgc tcggggagct gcaggagagc
      601 cagagtcgct tggaggctgc cactaaggaa tgccaggctc tggagggtcg ggcccgggcg
      661 gccagcgagc aggcgcggca gctggagagt gagcgcgagg cgctgcagca gcagcacagc
      721 gtgcaggtgg accagctgcg catgcagggc cagagcgtgg agaccgcgct ccgcatggag
      781 cgccaggccg cctcggagga gaagaggaag ctggcccagt tgcaggtggc ctatcaccag
      841 ctcttccaag aatacgacaa ccacatcaag agcagcg
//
